Monkey Lymph Nodes full length tissue cDNA is made of RNA extracted from freshly harvested tissues of single healthy normal cynomolgus monkey adult donor using classical guanidine isothiocyanate phenol: chloroform extraction method. RNA is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with oligo dT primer.
Quality Control:
The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also tested as template for PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.
Applications:
cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.
Packing/shipping:
Each cDNA sample is routinely shipped on dry ice in 1.5 ml vials (30ul each) and is enough for 30 reactions (30 PCR amplifications).