Immunization Grade Bovine Type II Collagen, Solution (2 mg/ml x 5 ml)
Product details
DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose, and Na2HPO4)
APPLICATION: Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for the induction of collagen-induced arthritis (CIA) in certain species and strains of experimental animals.
QUANTITY: 5 ml
FORM: 2 mg/ml solution in 0.05M acetic acid
SOURCE: Bovine articular cartilage
MOLECULAR WEIGHT: 300 kDa
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
STORAGE: -20°C in the dark
STABILITY: 2 years
NOTES: The maximum concentration of type II collagen in acidic buffer is 4 mg/ml. In order to neutralize this product, add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (NaCl final concentration: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.
NOTE: Although type II collagen does not form gel at low concentrations, Chondrex, Inc. recommends keeping the collagen solution on ice.
REFERENCES:
R. Trelstad, et al. Biochemistry 9: 4993-4998 (1970)
E. Miller, Biochemistry 10: 1652-1659 (1971)
D. Trentham, et al. J. Exp. Med. 146: 857-868 (1977)
P. Wooley, et al. J. Immunology 135: 2443-2451 (1985)