Sample Volume：50ul
Wavelength：450nm
Assay range：15 pg/ml - 480 pg/ml
Sensitivity：1 pg/ml
Standard：540 pg/ml
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to hbp, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for hbp is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain hbp and HRP conjugated hbp antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of hbp. You can calculate the concentration of hbp in the samples by comparing the OD of the samples to the standard curve.