Clone: 1G12
Size: 25 microgram
Applications: Immunohistochemistry, Western Blot, ELISA
Reactivity: Human
Synonyms: GPC3, OCI-5, GTR2-2, MXR7, OCI5, SGBS, SGBS1, DGSX, SGB, SDYS
Purification: Protein A/G Chromatography
Isotype: IgG1
Overview:
Glypican‐3 (GPC3) is a member of the glypican family of glycosyl phosphatidylinositol‐anchored cell‐surface heparan sulfate proteoglycans. The 1G12 monoclonal antibody has been used to assess GPC3 expression in malignant and nonmalignant liver tissue samples and for enzyme‐linked immunosorbent assay (ELISA) for detection of GPC3 in the serum. Using the 1G12 antibody, GPC3 has been identified as a useful tumor marker for the diagnosis of Hepatocellular Carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms’ tumor. In patients with HCC, GPC3 is over expressed in neoplastic liver tissue, but is undetectable in normal adult liver, benign liver, and the serum of healthy donors.
IMMUNOGEN: Balb/C mice immunized with 50 μg of a fragment containing the last 70 amino acids of the core protein.
SHIP CONDITIONS: Ship at ambient temperature, refrigerate upon arrival.
STORAGE: Product should be stored at 2‐8 degrees C.
STABILITY: Products are stable for one (1) year from purchase when stored properly.
DISTRIBUTION: Embryonic liver, hepatocellular carcinoma, Wilms' tumor, squamous cell carcinoma of the lung, germ cell tumors.
References
Capurro M, et al. Gastroenterology. 2003; 125:89-97.
Coston WMP, et al. Am J Surg Pathol. 2008; 32:433-44.
Kandil D, et al. Cancer. 2007; 111:316-22.
Zynger DL, et al. Hum Pathol. 2008; 39:224-30.
Kandil DH, et al. Adv Anat Pathol. 2009; 16:125-129.
Zynger DL, et al. Am J Surg Pathol. 2006; 30:1570-1575.