Operation Suggestion
1. strains preserved in glycerol medium 20%, received after the strain, please pick bacteria in 2x YT-N tablet (2xYT medium containing 100ug/ml nalidixic acid) on the line, 37centigradenbsp;after overnight incubation, again from single colony in the Minimal Medium plate line, 37 degrees can be used after overnight culture, in order to make the cilia fully expressed, conducive to phage infection;
2. the bacteria that grow on the Minimal Medium plate should not be used in the refrigerator for a long time.
3. Minimal Medium plate prepare method.