Product Overview:
The genotype of Ar.1193 Electroporation Competent Cell is Agrobacterium rhizogenes (carb R,str R,rif R) pRi1193 (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The Ar.1193 Agrobacterium rhizogenes strain contains pRi1193 agrobacterium-type Ri plasmid with a broad host range (leguminosae, solanaceae, etc.) and carbenicillin, streptomycin, rifampicin resistance. This competent cell is particularly suitable for the transformation of large plasmids.
Applications: Ar.1193 Electroporation Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.
Notes:
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Transformation efficiency:
Transformation efficiency of Ar.1193 Electroporation Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^5 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.
Storage: Store at - 80 °C for 12 months.