Western Blotting: Purified protein RVFG25-R-100 is available as
a control with RVFV11-S. Use at dilution of 1:50,000-1:100,000
ELISA: A450 of 1.0 obtained against RFVG25-R-10 at a dilution
of 125k
*Above dilutions are a suggestion and user’s must optimize based
on their own assays. This protein can be used for applications not
suggested above but has not been validated by ADI in other
applications.
Specificity & Cross-reactivity
RVFG25-R-100 has 59% homology with Gabek Forest virus
glycoprotein, 57% Karimabad virus glycoprotein, 55% Uriurana
virus glycoprotein, 54% Uriurana virus glycoprotein, and 53%
Punta Toro virus M protein.
References: Dessau M, Modis Y (January 2013). Proc. Natl.
Acad. Sci. U.S.A. 110 (5): 1696–701; Jup, P. G. (2002). Medical
and veterinary entomology 16 (3): 245–252; Turell, M. J (2007).
Journal of the American Mosquito Control Association 23 (4): 378–
382.