EvaGreen® Dye, 20X in Water - Trial size (1 mL)

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421,40 лв 421.40000000000003 BGN 421,40 лв

215,00 €

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    EvaGreen® Dye is a patented green fluorescent nucleic acid dye that is essentially non-fluorescent by itself, but becomes highly fluorescent upon binding to dsDNA.  It is ideal for a wide variety of applications including qPCR and DNA melt curve analysis, HRM®, LAMP, digital PCR, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), capillary gel electrophoresis, and more. The dye is provided at 20X (25 uM) in water.

    • Brighter and less inhibitory than SYBR® Green
    • Environmentally safe, non-mutagenic, and non-cytotoxic
    • Unsurpassed thermal stability, chemical stability, and photostability
    • Excellent compatibility with multiplex PCR
    • Directly visualize amplification products in a gel
    • Compatible with common qPCR instruments and enzyme systems

    The Unique Properties of EvaGreen® Dye

    EvaGreen® Dye was designed with a novel “release-on-demand” mechanism. In the absence of DNA, the dye assumes a conformation that is inactive. When DNA is available, the dye shifts to an active conformation that binds DNA to emit fluorescence. This property of the dye provides a unique mechanism to continuously supply the active form of the dye from an inactive reserve, as more DNA is produced during the PCR process. As a result, EvaGreen® Dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for digital PCR, real-time qPCR, HRM®, and multiplex qPCR by DNA melt curve analysis. This technology also makes it possible to significantly shorten the chain extension time, enabling the use of fast PCR protocols. 


    PRODUCT ATTRIBUTES

    Detection method/readout

    PCR/qPCR, HRM®

    Excitation/Emission

    500/530 nm (with DNA)

    Concentration

    20X (25 uM)

    Storage Conditions

    Store at -10 to -35 °C