Cellular Autophagy ELISA Kit (LC3-II Quantitation) 48T

https://www.gentaur.be/web/image/product.template/3271/image_1920?unique=bb60c10

1450,40 лв 1450.4 BGN 1450,40 лв

740,00 €

Not Available For Sale

    This combination does not exist.

    Terms and Conditions
    30-day money-back guarantee
    Shipping: 2-3 Business Days

    Cellular Autophagy ELISA Kit (LC3-II Quantitation) 48T

    Product details

    LC3 is a small 16-18 kDa protein that is found in 3 forms within cells. A pro-form of 125 amino acids becomes cleaved to a 120 amino acid form known as LC3-I. Upon binding to phosphatidylethanolamine (PE), the LC3-II form is generated. LC3-I is found mainly in the cytosol, and LC3-II is found mainly in the membrane fraction.

    Upon induction of autophagy by a stress signal such as amino acid starvation, the first step is the formation of autophagosomes.Both LC3-I and LC3-II are seen in healthy cells, but upon induction of autophagy the LC3-II proportion increases as it associates with the membranes of the autophagosomes.

    Our Autophagy ELISA Kit (LC3-II Quantitation) uses a selective permeabilization procedure to remove the cytosolic pro-LC3 and LC3-I and retain the autophagosome membrane-bound LC3-II from cells cultured in a 48-well plate. The cells are then probed with an anti-LC3 antibody, followed by an HRP-conjugated secondary antibody. Each kit provides sufficient reagents to perform up to 48 assays.