- Complete Expression System contains packaging plasmids, expression vector and GFP control vector for packaging of recombinant AAV serotype 2
- pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
- Cloning capacity of expression vector = 3 kb
The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells.
Recent Product Citations
- Tseng, S.J. et al. (2022). An acid degradable, lactate oxidizing nanoparticle formulation for non-small cell lung cancer virotherapy. Nano Today. doi: 10.1016/j.nantod.2022.101582.
- Hsieh, C.C. et al. (2022). Macrophage Distribution Affected by Virus-Encoded Granulocyte Macrophage Colony Stimulating Factor Combined with Lactate Oxidase. ACS Omega. doi: 10.1021/acsomega.2c03213.
- Kuwabara, Y. et al. (2020). Lionheart LincRNA alleviates cardiac systolic dysfunction under pressure overload. Commun Biol. 3(1):434. doi: 10.1038/s42003-020-01164-0.
- Okubo, Y. et al. (2019). Store-operated Ca2+ entry-dependent Ca2+ refilling in the endoplasmic reticulum in astrocytes. Biochem Biophys Res Commun. pii: S0006-291X(19)32326-5. doi: 10.1016/j.bbrc.2019.12.006.
- Robert, M.A. et al. (2018). Gene Transfer of ZMapp Antibodies Mediated by Recombinant Adeno-Associated Virus Protects Against Ebola Infections. Hum Gene Ther. 29(4):452-466. doi: 10.1089/hum.2017.101.