EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit - 96 assays

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1673,84 лв 1673.8400000000001 BGN 1673,84 лв

854,00 €

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    EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit - 96 assays

    We recommend using a newer version of this product: EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)



    The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA methyltransferase activity or inhibition at extremely fast speeds. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is suitable for measuring DNMT activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:

    • Very rapid procedure, which can be completed within 3 hours.
    • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
    • Strip microplate format makes the assay flexible: manual or high throughput analysis.
    • Extremely simple, reliable, and consistent assay conditions.

    Principle & Procedure
    The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is designed for measuring total DNMT activity (de novo, maintenance). In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. DNMT enzymes transfer a methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.