Use: For the qualitative determination of human anti-hepatitis B virus surface antibody (HBsAb) concentrations in serum and plasma.
Principle of the assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBsAg. Samples are pipetted into the wells with HBsAg conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HBsAb bound in the initial step. The color development is stopped and the intensity of the color is measured.