Immunization Grade Mouse Type II Collagen - 1 mg, lyophilized

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    Immunization Grade Mouse Type II Collagen - 1 mg, lyophilized

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    DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose, and Na2HPO4)

    APPLICATION: Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for the induction of collagen-induced arthritis (CIA) in certain species and strains of experimental animals.

    QUANTITY: 1 mg

    FORM: Lyophilized powder

    SOURCE: Mouse sternal cartilage

    MOLECULAR WEIGHT: 300 kDa

    PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans

    STORAGE: 4°C in the dark

    STABILITY: 2 years

    NOTES: Soluble in acidic buffer (maximum 4 mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve this product in a neutral buffer, first dissolve collagen in 0.01M or 0.05M acetic acid at 1-4 mg/ml and then add this collagen solution to the desired neutral buffer such as 2-10X TrisNaCl (NaCl final concentration: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.

    NOTE 1: To avoid fibril formation under neutral conditions, keep solution on ice.

    NOTE 2: The physicochemical property of type II collagen differs from type I collagen and type II collagen dissolved in a neutral buffer does not form a stable gel incubating at 37°C. In order to prepare a type II collagen gel, additional components such as type I collagen or other substances may be required. 

    REFERENCES:

    R. Trelstad, et al. Biochemistry 9: 4993-4998 (1970)

    E. Miller, Biochemistry 10: 1652-1659 (1971)

    D. Trentham, et al. J. Exp. Med. 146: 857-868 (1977)

    P. Wooley, et al. J. Immunology 135: 2443-2451 (1985)