Description: Purified, Staphylococcus aureus recombinant leukocidin-D (rLukD). The rLukD (tag free)
is expressed in E. coli and purified by FPLC. The
theoretical molecular weight of the protein is 36,889 Daltons.
Storage: 2-3 weeks at -20◦C, -80◦C long term
Size: 100 ug of protein is supplied in PBS at a
concentration of 2.224 mg/mL. Protein demonstrates a molecular weight of approximately 37 kDa.
Endotoxin: 4.232 Endotoxin units/mg (for Lot# 1707001)
Relevance: This protein may be used in functional
toxicity assays in combination with rLukE, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of rLukD protein using anti-PVL LukF polyclonal antibody (cat# 0312-002) in Western blotting.
Cytotoxicity assay: Cytotoxicity can be detected in
human neutrophils when used in combination with
LukE.