Recombinant Staphylococcus aureus Leukocidin-D (rLukD), Tag-free - 100 ug
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    Description: Purified, Staphylococcus aureus recombinant leukocidin-D (rLukD). The rLukD (tag free)
    is expressed in E. coli and purified by FPLC. The
    theoretical molecular weight of the protein is 36,889 Daltons.

    Storage: 2-3 weeks at -20◦C, -80◦C long term

    Size: 100 ug of protein is supplied in PBS at a
    concentration of 2.224 mg/mL. Protein demonstrates a molecular weight of approximately 37 kDa.

    Endotoxin: 4.232 Endotoxin units/mg (for Lot# 1707001)

    Relevance: This protein may be used in functional
    toxicity assays in combination with rLukE, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.

    Recommended Dilutions:
    ELISA: Assay-dependent dilution.
    WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of rLukD protein using anti-PVL LukF polyclonal antibody (cat# 0312-002) in Western blotting.

    Cytotoxicity assay: Cytotoxicity can be detected in
    human neutrophils when used in combination with