Endotoxin-Free Plasmid Miniprep Kit - 50 extractions
Cat. #: W2106-50 (50 reactions) when order from Fisher Sci. Cat.#: NC1492021
Storage: room temperature. After adding RNase A, store Buffer P1 at 2-8 oC, and it is stable for 6 months. Other buffers and RNase A stock solution can be stored for 12 months at room temperature.
Product Description (This product is for research use only.)
Endotoxin is a common contaminant in plasmid extraction. Because eukaryotic cells are very sensitive to endotoxin, the transfection efficiency will be greatly reduced if endotoxin is contained in the plasmid. This kit provides a simple, rapid, and efficient method for the extraction of endotoxin-free plasmids. The contaminations such as endotoxin, genome DNA, RNA, and proteins can be maximumly removed. The operation is simple and convenient.
This kit is suitable for the extraction of 1-5 ml bacterial cells. Based on conventional alkaline lysis method, the new
silicon membrane binds plasmid DNA efficiently and specifically. Each column can adsorb up to 40 μg of plasmid DNA.
Special buffer systems and endotoxin removal columns can remove impurities such as endotoxins and proteins. The
plasmids obtained from the kit are pure and stable and are particularly suitable for transfection experiments. The
plasmids and can also be used for downstream experiments such as DNA sequencing, PCR, PCRbased mutations, in
vitro transcription, transformation of bacteria, and restriction enzyme digestion. NOT included in the kit: 100%
ethanol; Isopropanol.