Origin: In vitro spontaneously transformed keratinocytes from histologically normal skin.
Species:Homo sapiens
Tissue: Skin
Morphology: Keratinocyte
Properties: Adherent monolayer
Patient: Male, Caucasian, 62 years of age.
Medium: Optimized DMEM (C0003-02) + 10% FBS
Subculture: Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X,X
CSF1PO: 9,11
D13S317: 10,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 11,12
vWA: 16,17
Related cells by Organ:
A431 cells
SK-Mel-28 cells
UACC-62 cells
C-32 cells
HaCaT cells
HaCaT Cells References:
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