DTT (Dithiothreitol) is a common reducing agent in biochemistry. Removal of DTT is required for Cys-based protein labeling and for disulfide bond formation in proteins. The Colorimetric DTT Assay Kit (Catalog number DTT100C) is designed for measurement of micromolar concentrations of DTT. The assay is based on measurement of light absorbance at 495 nm. The assay kit can be used for measurements DTT concentrations in biological samples, biochemical reactions and environmental water samples. The assay is compatible with common buffers. It is not compatible with NADH or NADPH.
The Colorimetric DTT Assay Kit (catalog number DTT100C) includes 1 ml of 10 x Buffer, 0.2 ml of 10 x
Reagent A, 0.2 ml of 10 x Reagent B and 1 ml of 10 x Reagent C. It is for measurement of 100 samples
using 96-well plates. Cuvettes may also be used for measurements.