Gene Name: HEY2
Detect Range: 0.312-20 ng/mL
Sensitivity: 0.039ng/mL
Target Protein Name: Human HEY2
Sample type: serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type: Sandwich ELISA Kit
ELISA Test Principle:
The microtiter plate provided in Human HEY2 ELISA KIT has been pre-coated with an Human HEY2 antibody specific to Human HEY2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human HEY2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HEY2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human HEY2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.