Detect Range: 78.1-5000pg/ml
Sensitivity: 46.9pg/ml
Target Protein Name: Mouse Rbbp7
Sample type: serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type: Sandwich ELISA Kit
ELISA Test Principle:
The microtiter plate provided in Mouse Histone- binding protein RBBP7, Rbbp7 ELISA KIT has been pre-coated with an Mouse Histone-binding protein RBBP7, Rbbp7 antibody specific to Mouse Rbbp7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rbbp7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rbbp7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rbbp7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.