Bacterial Resistance: Kanamycin
Growth Strain: DH5α
Expression: Bacterial
Use: pET Plasmid
Promoter: T7/lac
Replicator: ColE1, ori, F1, ori
Terminator: T7 Terminator
Plasmid classification: Escherichia coli vector, PET series of expressed plasmids
Plasmid size: 5633bp
Plasmid labels: N-6 * His, N-Thrombin, N-SUMO, C-6 * His
Prokaryotic resistance: kanamycin Kan
Clone strain: DH5 alpha
Culture conditions: 37 ℃, aerobic, LB
Expression host: BL21 (DE3)
Culture conditions: 37 ℃, aerobic, LB
Induction: IPTG or lactose and its analogues
Primers for 5'sequencing: T7: TAATACGACTCACTATAGGG
Primers for 3'sequencing: T7-ter: TGCTAGTTATTGCTCAGCGG