PAX01- COMK plasmid - 2 ug

https://www.gentaur.be/web/image/product.template/2905/image_1920?unique=01dd35d

885,92 лв 885.9200000000001 BGN 885,92 лв

452,00 €

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    pAX01-ComK

    Catalog No. PVT11299
    Packing 2ug

     

    pAX01-ComK Information

    Note: integrated xylose induction

    Promoter: xylA

    Replicator: repB

    Clone strain: DH5a

    Culture condition: 37℃

    Host: Bacillus subtilis

    Purpose of plasmid: protein expression

    Fragment Type: ORF

    Fragment species: Bacillus subtilis

    Prokaryotic resistance: Amp

    Filter Tag: Ery

     

    pAX01-ComK Sequence

    ggggtgatgtcaaagcttgaaaaaacgcacgtaacaaaagcaaaatttatgctccatgggggagactacaaccccgatcagtggctggatcggcccgatattttagctgacgatatcaaactgatgaagctttctcatacgaatacgttttctgtcggcatttttgcatggagcgcacttgagccggaggagggcgtatatcaatttgaatggctggatgatatttttgagcggattcacagtataggcggccgggtcatattagcaacgccgagcggagcccgtccggcctggctgtcgcaaacctatccggaagttttgcgcgtcaatgcctcccgcgtcaaacagctgcacggcggaaggcacaaccactgcctcacatctaaagtctaccgagaaaaaacacggcacatcaaccgcttattagcagaacgatacggacatcacccggcgctgttaatgtggcacatttcaaacgaatacgggggagattgccactgtgaatcgatgcggccgctctagagcaacgttcttgccattgctgcataaaaaacgcccggcggcaaccgagcgttctgaattaattaatcatcgggaagatcttcatcaccgaaacgcggcaggcagctctagagttaacaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagctagagcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctcaagctagagagtcattaccagatctcactgcagagatccccgggtctagagtctagggacctctttagctccttggaagctgtcagtagtatacctaataatttatctacattccctttagtaacgtgtaactttccaaatttacaaaaGCGACTCATAGAATTATTTCCTCCCGTTAAATAATAGATAACTATTAAAAATAGACAATACTTGCTCATAAGTAACGGTCTTAAATTGTTTACTTTGGCGTGTTTCATTGCTTGATGAAACTGATTTTTAGTAAACAGTTGACGATATTCTCGATTGACCCATTTTGAAACAAAGTACGTATATAGCTTCCAATATTTATCTGGAACATCTGTGGTATGGCGGGTAAGTTTTATTAAGACACTGTTTACTTTTGGTTTAGGATGAAAGCATTCCGCTGGCAGCTTAAGCAATTGCTGAATCGAGACTTGAGTGTGCAAGAGCAACCCTAGTGTTCGGTGAATATCCAAGGTACGCTTGTAGAATCCTTCTTCAACAATCAGATAGATGTCAGACGCATGGCTTTCAAAAACCACTTTTTTAATAATTTGTGTGCTTAAATGGTAAGGAATACTCCCAACAATTTTATACCTCTGTTTGTTAGGGAATTGAAACTGTAGAATATCTTGGTGAATTAAAGTGACACGAGTATTCAGTTTTAATTTTTCTGACGATAAGTTGAATAGATGACTGTCTAATTCAATAGACGTTACCTGTTTACTTATTTTAGCCAGTTTCGTCGTTAAATGCCCTTTACCTGTTCCAATTTCGTAAACGGTATCGGTTTCTTTTAAATTCAATTGTTTTATTATTTGGTTGAGTACTTTTTCACTCGTTAAAAAGTTTTGAGAATATTTTATATTTTTGTTCATGTAATCACTCCTTCTTAATTACAAATTTTTAGCATCTAATTTAACTTCAATTCCTATTATACAAAATTTTAAGATACTGCACTATCAACACACTCTTAAGTTTGCTTCTAAGTCTTATTTCCATAACTTCTTTTACGTTTCCGCCATTCTTTGCTGTTTCGATTTTTATGATATGGTGCAAGTCAGCACGAACACGAACCGTCTTATCTCCCATTATATCTTTTTTTGCACTGATTGGTGTATCATTTCGTTTTTCTTTTTGTGCTAGAGGATCAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTGCGGCCGCCCGCGGCTATTTTTCTAATACCGTTCCCCGAGCTCACGCAAAATAAAATCATAAATCATCTTCGTCCGCTCTTCTTTCGGGTACAGCATAAATTCCTGTCTTTTCGGCACGCGGTGGTCGATTCTGTCTTGGAATTTGGTTCTGAGCCACGCTGTTCGGTATACCTGGTTCTCGAACGAATTATAAGAGATCGGCAGCTCCATCGTTTTCCCATTGGAAAACGTCACTTCCGTATCGTCGAATTCAGTCGCTTTGAATTCTTTTACATGCACATGGGAAATCCAGCCGCATTGGGGTCTTGTCGAAGAAAGTGTAGGGAATAAAAAGATTTGGTTCGAAGGGTCCACCATGATCGGCGGCTTGTGTGAAATTTTTGTCACTTCATAAGTTCCTGCTTTTCTTCCCGCATAGCTTGATCCAAAAAATCGGCAGCTTCTGTCGACAATTTGCAGCGGCTTCATGTTTACATAAAACACGCAATCTTTTTCAATAATTTTGGAACAGATTTTGCCGTCTATTTCTTCTGGCAGCACGGCAATTGTTGCGCCGTTCACTTCATACGATTCTAAAGGTGCGTCTGTTTTCTGACTCATATTATGGCCTCCTTTGGATCCcatttccccctttgatttttagatatcactagtttggaccatttgtcatttccccctttgatttaagtgaacaagtttatccatcaactatcttaattgagttagtttgtttatccaataaactaactttatctcatcatatacaaaataaatgtttatttcaatgttttttttagaaaatttagttataatattagatatgatacttttaaatatctaattcaagcttcaaaaaacaccaacttagttcggtggataaacaaaggagtggttattattcaaattgcagatcaagctttagtaaaaaaaatgaatcaaaaattaatattagatgaaattttgaagaactcccctgtctccagggcaactctctctgagattacaggattaaacaagtctactgtctcctctcaagtaaatacactgcttgaaaaagattttatttttgaaattggggcagggcaatctagaggcggcagaagacctgtaatgcttgtttttaataagaatgcaggctactcgattggtattgatataggagtcgactatcttaacggaattctaaccgacttagaaggaaatattattctcgagaagacttctgacttgtctagttcttccgctagtgaagtaaaagagattttatttgcacttattcatggttttgtaacccatatgcctgagtccccttatggtctagtcggaataggaatttgtgttccaggccttgtagatcgtcatcagcaaattattttcatgcctaacttaaattggaatatcaaagatttgcagtttttaattgagagtgagtttaatgttccggtttttgttgaaaatgaagctaatgcaggagcatacggtgaaaaagtatttggtatacaaaaaactatgaaaacatcgtttacatcagtattaatatcggaattggaactggacttgttattaacaacgaattgtataaggtgttcagggtttttctggggaaatgggtcatatgacgatagattttaatggacccaaatgcagctgtggaaatcgaggcgttgggaattatatgcttctgaaaaagcgttactggcttcgctctctaaagaagaaaagaatatttctcgaaaagagattgtgaacgcgcaaataaaaatgatgtagaaatgttaaatgcacttcaaaactttggcttttatatcggaattggattaaccaatatcttaatacatttgatatagaagctgttatcttgagaaatcatataattgaatctcatcccattgttttaaatacgattaaaaacgaagtttcttctagagtccattctcatttagacaataaatgtgaactattgccttcttcgttaggaaaaaatgcacctgctttaggagcggtttctatcgttattgattcttttttaagtgttacccctataagttaggagctccccgggacgttcttgccattgctgcataaaaaacgcccggcggcaaccgagcgttctgaattaattaatcatcgcgactgcagagatatcgatttcaagctatatttggagttgagcctcttgaaacggacaccctgtatccgaaggatcgaaacgctgtcagctaccgcagccaaatatatgaaatgaaggattatgcaaccgtgattgatgtaaagacagcttcagtggaagcggtgtatcaagaagatttttatgcgcgcacgccagcggtcacaagccatgagtatcagcagggcaaggcgtattttatcggcgcgcgtttggaggatcaatttcagcgtgatttctatgagggtctgatcacagacctgtctctctctccagtttttccggttcggcacggaaaaggcgtctccgtacaagcgaggcaggatcaggacaatgattatatttttgtcatgaatttcacggaagaaaaacagctggtcacgtttgatcagagtgtgaaggacataatgacaggagacatattgtcaggcgacctgacgatggaaaagtatgaagtgagaattgtcgtaaacacacattagcccatcattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccggg......
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    Caution:
    1.  This product is FOR RESEARCH USE ONLY!
    2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
    3.  Shipping temperature is 2-8℃.