This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double
antibody sandwich technology to assay the Guinea pig Growth hormone (GH). Add Growth
hormone(GH)to the wells, which are pre-coated with Growth hormone (GH) monoclonal
antibody and then incubate. After that, add anti GH antibodies labeled with biotin to unite
with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after
incubation and washing. Add substrate A and B. Then the solution will turn blue and change
into yellow with the effect of acid. The shades of solution and the concentration of Guinea pig
Growth hormone (GH) are positively correlated.
ASSAY RANGE: 1pg/ml - 200pg/ml
SENSITIVITY: 0.39pg/ml
ASSAY PROCEDURE SUMMARY:
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at
37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10minutes.
6. Calculate.